Hydrodynamic analysis of synthetic and bio-macromolecules

Complementary analytical techniques: Analytical ultracentrifugation, intrinsic viscosity, and light scattering

Presenters: Ivo Nischang, Friedrich Schiller University Jena, Germany and Igor Perevyazko, St. Petersburg State University, Russia

Description

  1. Conformational properties and solution behavior of macromolecules – basics: Molar mass, hydrodynamic size, end-to-end distance of polymer chains, conformational state (hard sphere, random coil, rigid rod), equilibrium rigidity, and diameter of polymer chains, dilute and semi-dilute polymer solutions, solvent quality 

  2. Complementary / orthogonal hydrodynamic methods:
    • Viscometry of macromolecules: Intrinsic viscosity and absolute (dynamic) viscosity, degree of dilution, viscometric parameters, charged and neutral macromolecules
    • Sedimentation velocity analysis of macromolecules: Heterogeneity of samples, LAMM equation modelling, concentration dependence of sedimentation coefficient and frictional ratio, Gralen coefficient, molar mass estimations, charged and neutral macromolecules
    • Translational diffusion measurements of macromolecules: Measurements of diffusion by synthetic boundaries, diffusion coefficients by dynamic light scattering (DLS), concentration dependences of diffusion coefficients, hydrodynamic radii / diameters (Stokes-Einstein)
  3. Hydrodynamic invariants: Consistency of the experimental data: Hydrodynamic invariant, A0, and sedimentation parameter, βs
  4. Complementary physical methods - separations and light scattering 
Size exclusion chromatography (SEC), asymmetrical flow field-flow fractionation (AF4), multi-angle laser light scattering (MALLS) – molar masses, radii of gyration and their distributions
  5. Absolute molar mass and hydrodynamic size in solution
  6. Conformation and conformational characteristics: Macromolecular shape and conformation in solution, Kuhn-Mark- Houwink-Sakurada scaling relationships, evaluation of equilibrium rigidity and diameter of polymer chains
  7. Strategy to study solution properties of synthetic and bio- macromolecular samples of unknown properties

This workshop is offered during sessions 5-6 and a repeat during sessions 9-10.