Fluorescence Spectroscopy: a complement to AUC

Presenters: Sandy Ross and Harmen Steele, University of Montana


This workshop is an introduction to fluorescence methods used in the life sciences and nanotechnology to study questions about dynamics and hydrodynamics. We will review the basics of steady-state and time-resolved fluorescence measurements. Topics to be covered include: Förster resonance energy transfer (FRET), which is often used to study conformational change and distances between 1-10 nm; fluorescence anisotropy (steady-state and time-resolved), which can be used to study nanosecond-timescale dynamics of membranes and macromolecules, such as DNA and proteins; and fluorescence correlation spectroscopy (FCS), which can be used to determine translational diffusion coefficients of biological molecules and their assemblies, including membranes.

This workshop is presented on Saturday during session 1, and a repeat of the same workshop is offered on Sunday during session 6.